smac mimetics degrade ciap1 Search Results


smac mimetic bv6  (SMAC Corp)
90
SMAC Corp smac mimetic bv6
The SMAC mimetic <t>BV6</t> enhances TNF-induced procaspase-8 processing. (A) HCT116-PI3Kmut cells were pretreated or not with 10 μM BV6 for 2 h and were then stimulated with increasing concentrations of TNF for 10 min. Phosphorylation and degradation of IκBα and cIAP1 expression were analyzed by Western blotting. (B) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (KO) cells were again pretreated with 10 μM for 2 h and then stimulated overnight with 200 ng/ml of TNF, TRAIL, or Fc-CD95L. IL8 production was determined by ELISA. Shown are results of three or four independent experiments. * p < 0.05; n.s., not significant. (C) BV6-pretreated (2 h, 10 μM) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (TRAF2-KO) cells were stimulated with the indicated concentrations of TNF for 6 h in the presence of 2.5 μg/ml CHX and subjected to Western blot analysis.
Smac Mimetic Bv6, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smac mimetics antagonize ciap1 2  (Mimetics)
86
Mimetics smac mimetics antagonize ciap1 2
The SMAC mimetic <t>BV6</t> enhances TNF-induced procaspase-8 processing. (A) HCT116-PI3Kmut cells were pretreated or not with 10 μM BV6 for 2 h and were then stimulated with increasing concentrations of TNF for 10 min. Phosphorylation and degradation of IκBα and cIAP1 expression were analyzed by Western blotting. (B) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (KO) cells were again pretreated with 10 μM for 2 h and then stimulated overnight with 200 ng/ml of TNF, TRAIL, or Fc-CD95L. IL8 production was determined by ELISA. Shown are results of three or four independent experiments. * p < 0.05; n.s., not significant. (C) BV6-pretreated (2 h, 10 μM) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (TRAF2-KO) cells were stimulated with the indicated concentrations of TNF for 6 h in the presence of 2.5 μg/ml CHX and subjected to Western blot analysis.
Smac Mimetics Antagonize Ciap1 2, supplied by Mimetics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ciap1/2 smac mimetic  (SMAC Corp)
90
SMAC Corp ciap1/2 smac mimetic
The SMAC mimetic <t>BV6</t> enhances TNF-induced procaspase-8 processing. (A) HCT116-PI3Kmut cells were pretreated or not with 10 μM BV6 for 2 h and were then stimulated with increasing concentrations of TNF for 10 min. Phosphorylation and degradation of IκBα and cIAP1 expression were analyzed by Western blotting. (B) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (KO) cells were again pretreated with 10 μM for 2 h and then stimulated overnight with 200 ng/ml of TNF, TRAIL, or Fc-CD95L. IL8 production was determined by ELISA. Shown are results of three or four independent experiments. * p < 0.05; n.s., not significant. (C) BV6-pretreated (2 h, 10 μM) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (TRAF2-KO) cells were stimulated with the indicated concentrations of TNF for 6 h in the presence of 2.5 μg/ml CHX and subjected to Western blot analysis.
Ciap1/2 Smac Mimetic, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mimetic compound lcl161  (SMAC Corp)
90
SMAC Corp mimetic compound lcl161
Depletion of cIAP1 using Smac Mimetic (LC161) or siRNA, sensitizes TNBCs to DNA damage-induced apoptosis in drug resistant PAR-4 deficient cells. ( a ) Par-4 expression in the indicated TNBC cells was silenced as shown in (Fig. ). Cells were pre-treated with cIAP1 antagonist <t>LCL161</t> (0,5 μM) for 1 h and then stimulated with Etoposide (100 μM) for 16 h. Cell lysates were analysed by immunoblotting using the indicated antibodies. ( b ) TNBCs from ( a ) were prepared for cell cycle analysis and the sub-G1 population was measured via flow cytometry. The corresponding histograms are presented in Supplementary Fig. . Average values from three independent experiments are shown. Error bars indicate ± SD. ( c ) PAR-4 and cIAP1 expression were silenced in BT-20 and MDA-MB-468 cells by using specific siRNAs (siPAR-4 #1, siPAR-4 #2 and sicIAP1). After incubation for 48 h cells were stimulated with Etoposide (100 μM) for 16 h and cell lysates were analysed by immunoblotting using the indicated antibodies. ( a , c ) Full-length blots are presented in Supplementary Fig. .
Mimetic Compound Lcl161, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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second mitochondrial activator of caspases (smac) mimetic  (SMAC Corp)
90
SMAC Corp second mitochondrial activator of caspases (smac) mimetic
Depletion of cIAP1 using Smac Mimetic (LC161) or siRNA, sensitizes TNBCs to DNA damage-induced apoptosis in drug resistant PAR-4 deficient cells. ( a ) Par-4 expression in the indicated TNBC cells was silenced as shown in (Fig. ). Cells were pre-treated with cIAP1 antagonist <t>LCL161</t> (0,5 μM) for 1 h and then stimulated with Etoposide (100 μM) for 16 h. Cell lysates were analysed by immunoblotting using the indicated antibodies. ( b ) TNBCs from ( a ) were prepared for cell cycle analysis and the sub-G1 population was measured via flow cytometry. The corresponding histograms are presented in Supplementary Fig. . Average values from three independent experiments are shown. Error bars indicate ± SD. ( c ) PAR-4 and cIAP1 expression were silenced in BT-20 and MDA-MB-468 cells by using specific siRNAs (siPAR-4 #1, siPAR-4 #2 and sicIAP1). After incubation for 48 h cells were stimulated with Etoposide (100 μM) for 16 h and cell lysates were analysed by immunoblotting using the indicated antibodies. ( a , c ) Full-length blots are presented in Supplementary Fig. .
Second Mitochondrial Activator Of Caspases (Smac) Mimetic, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smac mimetic birinapant  (SMAC Corp)
90
SMAC Corp smac mimetic birinapant
Depletion of cIAP1 using Smac Mimetic (LC161) or siRNA, sensitizes TNBCs to DNA damage-induced apoptosis in drug resistant PAR-4 deficient cells. ( a ) Par-4 expression in the indicated TNBC cells was silenced as shown in (Fig. ). Cells were pre-treated with cIAP1 antagonist <t>LCL161</t> (0,5 μM) for 1 h and then stimulated with Etoposide (100 μM) for 16 h. Cell lysates were analysed by immunoblotting using the indicated antibodies. ( b ) TNBCs from ( a ) were prepared for cell cycle analysis and the sub-G1 population was measured via flow cytometry. The corresponding histograms are presented in Supplementary Fig. . Average values from three independent experiments are shown. Error bars indicate ± SD. ( c ) PAR-4 and cIAP1 expression were silenced in BT-20 and MDA-MB-468 cells by using specific siRNAs (siPAR-4 #1, siPAR-4 #2 and sicIAP1). After incubation for 48 h cells were stimulated with Etoposide (100 μM) for 16 h and cell lysates were analysed by immunoblotting using the indicated antibodies. ( a , c ) Full-length blots are presented in Supplementary Fig. .
Smac Mimetic Birinapant, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smac+mimetics+degrade+ciap1/pm31303383-268-13-12?v=SMAC+Corp
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ciap1  (Mimetics)
86
Mimetics ciap1
Depletion of cIAP1 using Smac Mimetic (LC161) or siRNA, sensitizes TNBCs to DNA damage-induced apoptosis in drug resistant PAR-4 deficient cells. ( a ) Par-4 expression in the indicated TNBC cells was silenced as shown in (Fig. ). Cells were pre-treated with cIAP1 antagonist <t>LCL161</t> (0,5 μM) for 1 h and then stimulated with Etoposide (100 μM) for 16 h. Cell lysates were analysed by immunoblotting using the indicated antibodies. ( b ) TNBCs from ( a ) were prepared for cell cycle analysis and the sub-G1 population was measured via flow cytometry. The corresponding histograms are presented in Supplementary Fig. . Average values from three independent experiments are shown. Error bars indicate ± SD. ( c ) PAR-4 and cIAP1 expression were silenced in BT-20 and MDA-MB-468 cells by using specific siRNAs (siPAR-4 #1, siPAR-4 #2 and sicIAP1). After incubation for 48 h cells were stimulated with Etoposide (100 μM) for 16 h and cell lysates were analysed by immunoblotting using the indicated antibodies. ( a , c ) Full-length blots are presented in Supplementary Fig. .
Ciap1, supplied by Mimetics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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Image Search Results


The SMAC mimetic BV6 enhances TNF-induced procaspase-8 processing. (A) HCT116-PI3Kmut cells were pretreated or not with 10 μM BV6 for 2 h and were then stimulated with increasing concentrations of TNF for 10 min. Phosphorylation and degradation of IκBα and cIAP1 expression were analyzed by Western blotting. (B) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (KO) cells were again pretreated with 10 μM for 2 h and then stimulated overnight with 200 ng/ml of TNF, TRAIL, or Fc-CD95L. IL8 production was determined by ELISA. Shown are results of three or four independent experiments. * p < 0.05; n.s., not significant. (C) BV6-pretreated (2 h, 10 μM) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (TRAF2-KO) cells were stimulated with the indicated concentrations of TNF for 6 h in the presence of 2.5 μg/ml CHX and subjected to Western blot analysis.

Journal: Frontiers in Immunology

Article Title: TRAF2 Controls Death Receptor-Induced Caspase-8 Processing and Facilitates Proinflammatory Signaling

doi: 10.3389/fimmu.2019.02024

Figure Lengend Snippet: The SMAC mimetic BV6 enhances TNF-induced procaspase-8 processing. (A) HCT116-PI3Kmut cells were pretreated or not with 10 μM BV6 for 2 h and were then stimulated with increasing concentrations of TNF for 10 min. Phosphorylation and degradation of IκBα and cIAP1 expression were analyzed by Western blotting. (B) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (KO) cells were again pretreated with 10 μM for 2 h and then stimulated overnight with 200 ng/ml of TNF, TRAIL, or Fc-CD95L. IL8 production was determined by ELISA. Shown are results of three or four independent experiments. * p < 0.05; n.s., not significant. (C) BV6-pretreated (2 h, 10 μM) HCT116-PI3Kmut (–) and HCT116-PI3Kmut-TRAF2 KO (TRAF2-KO) cells were stimulated with the indicated concentrations of TNF for 6 h in the presence of 2.5 μg/ml CHX and subjected to Western blot analysis.

Article Snippet: Nevertheless, TRAF2 deficiency and cIAP1 depletion with the SMAC mimetic BV6 come along with enhanced DR-induced processing of procaspase-8 ( , ).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Depletion of cIAP1 using Smac Mimetic (LC161) or siRNA, sensitizes TNBCs to DNA damage-induced apoptosis in drug resistant PAR-4 deficient cells. ( a ) Par-4 expression in the indicated TNBC cells was silenced as shown in (Fig. ). Cells were pre-treated with cIAP1 antagonist LCL161 (0,5 μM) for 1 h and then stimulated with Etoposide (100 μM) for 16 h. Cell lysates were analysed by immunoblotting using the indicated antibodies. ( b ) TNBCs from ( a ) were prepared for cell cycle analysis and the sub-G1 population was measured via flow cytometry. The corresponding histograms are presented in Supplementary Fig. . Average values from three independent experiments are shown. Error bars indicate ± SD. ( c ) PAR-4 and cIAP1 expression were silenced in BT-20 and MDA-MB-468 cells by using specific siRNAs (siPAR-4 #1, siPAR-4 #2 and sicIAP1). After incubation for 48 h cells were stimulated with Etoposide (100 μM) for 16 h and cell lysates were analysed by immunoblotting using the indicated antibodies. ( a , c ) Full-length blots are presented in Supplementary Fig. .

Journal: Scientific Reports

Article Title: PAR-4 overcomes chemo-resistance in breast cancer cells by antagonizing cIAP1

doi: 10.1038/s41598-019-45209-9

Figure Lengend Snippet: Depletion of cIAP1 using Smac Mimetic (LC161) or siRNA, sensitizes TNBCs to DNA damage-induced apoptosis in drug resistant PAR-4 deficient cells. ( a ) Par-4 expression in the indicated TNBC cells was silenced as shown in (Fig. ). Cells were pre-treated with cIAP1 antagonist LCL161 (0,5 μM) for 1 h and then stimulated with Etoposide (100 μM) for 16 h. Cell lysates were analysed by immunoblotting using the indicated antibodies. ( b ) TNBCs from ( a ) were prepared for cell cycle analysis and the sub-G1 population was measured via flow cytometry. The corresponding histograms are presented in Supplementary Fig. . Average values from three independent experiments are shown. Error bars indicate ± SD. ( c ) PAR-4 and cIAP1 expression were silenced in BT-20 and MDA-MB-468 cells by using specific siRNAs (siPAR-4 #1, siPAR-4 #2 and sicIAP1). After incubation for 48 h cells were stimulated with Etoposide (100 μM) for 16 h and cell lysates were analysed by immunoblotting using the indicated antibodies. ( a , c ) Full-length blots are presented in Supplementary Fig. .

Article Snippet: Indeed, depleting cIAP1 with the Smac mimetic compound LCL161 completely abrogated DNA damage-induced resistance mediated through the loss of PAR-4 (Fig. ).

Techniques: Expressing, Western Blot, Cell Cycle Assay, Flow Cytometry, Incubation